Deirdre A Roberts

US Patent:

60201487, Feb 1, 2000

Filed:

Jun 18, 1997

Appl. No.:

8/877963

Inventors:

Rosemarie Osborne - Oxford OH
Mary Ann Perkins - Cincinnati OH
Deirdre Anne Roberts - Loveland OH

Assignee:

The Procter & Gamble Company - Cincinnati OH

International Classification:

C12Q 132

US Classification:

435 26

Abstract:

A technique for in vitro testing of ocular and dermal irritants is disclosed. The process involves the topical application of liquid, solid granular or gel-like materials (e. g. cosmetics) to a cell culture and then evaluating the cytotoxicity of the material. Cell cultures of human skin without a stratum corneum and having a histologic similarity to the eye are used. Irritation is evaluated by measuring cell viability using MTT assay (based on the reduction of a tetrazolium dye by functional mitochondria), or release of LDH or PGE. sub. 2. A unique method of applying test materials which are not water soluble is described.