David A Ornelles

US Patent:

20030082811, May 1, 2003

Filed:

Jun 29, 2001

Appl. No.:

09/895940

Inventors:

Joseph Orlando - Winston-Salem NC, US
David Ornelles - Winston-Salem NC, US

International Classification:

C12N015/861
C12N007/00
C12N005/08

US Classification:

435/456000, 435/235100, 435/369000

Abstract:

A method of packaging a recombinant viral vector is carried out by: (a) providing a packaging cell, the packaging cell containing and expressing a nucleic acid encoding a mutant adenovirus E4orf6 protein, the E4orf6 protein containing at least one mutation that renders the protein non-toxic to the host cell; (b) transfecting or infecting the packaging cell with a nucleic acid that encodes a recombinant viral vector (e.g., an adenovirus vector or an adeno-associated virus vector), where the vector lacks a functional gene encoding E4orf6 protein; (c) culturing the transfected cells; and then (d) collecting packaged recombinant viral vector from the cultured cells. Nucleic acids, vectors and packaging cells used for carrying out the methods, as well as proteins utilized in the methods, are also described.