David Jeruzalmi from 1485 5Th Ave APT 14F, New York, NY 10035, age 60, Phone: 212 360-1925

Us Patents

Enzyme Derived From Thermophilic Organisms That Functions As A Chromosomal Replicase, And Preparation And Uses Thereof
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US Patent:

7556958, Jul 7, 2009
Filed:

Aug 18, 2000
Appl. No.:

09/642218
Inventors:

Olga Yurieva - New York NY, US
John Kuriyan - Riverdale NY, US
Michael E. O'Donnell - Hastings-on-Hudson NY, US
David Jeruzalmi - New York NY, US
Assignee:

The Rockefeller University - New York NY
International Classification:

C12N 1/20
C12N 9/12
C07H 21/04
US Classification:

4352523, 435194, 435183, 435 6, 4353201, 536 231, 536 232, 536 237
Abstract:

A DNA Polymerase has been identified in a thermophile that functions as a chromosomal replicase. The specific enzyme is a holoenzyme III that has been identified in , and corresponds to Polymerase III in. The genes and the polypeptides corresponding to. γ, τ, ε, α and β subunits that they encode are disclosed, as are probes, vectors, methods of preparation and the methods of use. The enzymes of the present invention and their components are particularly well suited for use in procedures for the preparation of DNA, such as PCR, because of the speed and accuracy that they are able to achieve.

Enzyme Derived From Thermophilic Organisms That Functions As A Chromosomal Replicase, And Preparation And Uses Thereof
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US Patent:

7803596, Sep 28, 2010
Filed:

Jun 4, 2009
Appl. No.:

12/478741
Inventors:

Olga Yurieva - New York NY, US
John Kuriyan - Riverdale NY, US
Michael E. O'Donnell - Hastings-on-Hudson NY, US
David Jeruzalmi - New York NY, US
Assignee:

The Rockefeller University - New York NY
International Classification:

C12N 9/12
C12N 1/20
US Classification:

435194, 435183, 4352523, 4353201, 536 231, 536 232, 536 233
Abstract:

A DNA Polymerase has been identified in a thermophile that functions as a chromosomal replicase. The specific enzyme is a holoenzyme III that has been identified in and corresponds to Polymerase III in. The genes and the polypeptides corresponding to T. th. γ, τ, ε, α and β subunits that they encode are disclosed, as are probes, vectors, methods of preparation and the methods of use. The enzymes of the present invention and their components are particularly well suited for use in procedures for the preparation of DNA, such as PCR, because of the speed and accuracy that they are able to achieve.

Nucleic Acid Encoding Bacillus Stearothermophilus Delta Polymerase Subunit
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US Patent:

20040038289, Feb 26, 2004
Filed:

Sep 25, 2003
Appl. No.:

10/671403
Inventors:

Michael O'Donnell - Hastings-on-Hudson NY, US
Alexander Yuzhakov - Malden MA, US
Olga Yurieva - New York NY, US
David Jeruzalmi - Cambridge MA, US
Irina Bruck - New York NY, US
John Kuriyan - Berkeley CA, US
International Classification:

C12Q001/68
C07H021/04
C12N009/22
C12P021/02
C12N001/21
C12N015/74
US Classification:

435/006000, 435/069100, 435/199000, 435/252310, 435/320100, 536/023200
Abstract:

The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polymerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.

Nucleic Acid Encoding Thermotoga Maritima Delta Prime Polymerase Subunit
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US Patent:

20040038290, Feb 26, 2004
Filed:

Sep 25, 2003
Appl. No.:

10/671419
Inventors:

Michael O'Donnell - Hastings-on-Hudson NY, US
Alexander Yuzhakov - Malden MA, US
Olga Yurieva - New York NY, US
David Jeruzalmi - Cambridge MA, US
Irina Bruck - New York NY, US
John Kuriyan - Berkeley MA, US
International Classification:

C12Q001/68
C07H021/04
C12N009/22
C12P021/02
C12N005/06
US Classification:

435/006000, 435/069100, 435/199000, 435/320100, 435/252300, 536/023200
Abstract:

The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polymerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.

Nucleic Acid Encoding Bacillus Stearothermophilus Tau Polymerase Subunit
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US Patent:

20040043414, Mar 4, 2004
Filed:

Sep 25, 2003
Appl. No.:

10/670844
Inventors:

Michael O'Donnell - Hastings-on- Hudson NY, US
Alexander Yuzhakov - Malden MA, US
Olga Yurieva - New York NY, US
David Jeruzalmi - Cambridge MA, US
Irina Bruck - New York NY, US
John Kuriyan - Berkeley CA, US
International Classification:

C12Q001/68
C07H021/04
C12N009/22
C12P021/02
C12N005/06
C12N015/74
US Classification:

435/006000, 435/069100, 435/199000, 435/252310, 435/320100, 536/023200
Abstract:

The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polymerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.

Nucleic Acid Encoding Aquifex Aeolicus Delta Prime Polymerase Subunit
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US Patent:

20040043415, Mar 4, 2004
Filed:

Sep 25, 2003
Appl. No.:

10/671134
Inventors:

Michael O'Donnell - Hastings-on-Hudson NY, US
Alexander Yuzhakov - Malden MA, US
Olga Yurieva - New York NY, US
David Jeruzalmi - Cambridge MA, US
Irina Bruck - New York NY, US
John Kuriyan - Berkeley CA, US
International Classification:

C12Q001/68
C07H021/04
C12N009/22
C12P021/02
C12N005/06
C12N015/74
US Classification:

435/006000, 435/069100, 435/199000, 435/252300, 435/320100, 536/023200
Abstract:

The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polymerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.

Thermotoga Maritima Delta Prime Polymerase Subunit And Use Thereof
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US Patent:

20040048309, Mar 11, 2004
Filed:

Sep 26, 2003
Appl. No.:

10/673098
Inventors:

Michael O'Donnell - Hastings-on-Hudson NY, US
Alexander Yuzhakov - Malden MA, US
Olga Yurieva - New York NY, US
David Jeruzalmi - Cambridge MA, US
Irina Bruck - New York NY, US
John Kuriyan - Riverdale NY, US
International Classification:

C12Q001/68
C07H021/04
C12N009/22
US Classification:

435/006000, 435/069100, 435/199000, 435/320100, 435/252300, 536/023200
Abstract:

The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polymerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.

Bacillus Stearothermophilus Polc Polymerase Subunit And Use Thereof
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US Patent:

20040077012, Apr 22, 2004
Filed:

Sep 26, 2003
Appl. No.:

10/672638
Inventors:

Michael O'Donnell - Hastings-on-Hudson NY, US
Alexander Yuzhakov - Malden MA, US
Olga Yurieva - New York NY, US
David Jeruzalmi - Cambridge MA, US
Irina Bruck - New York NY, US
John Kuriyan - Berkeley CA, US
International Classification:

C12Q001/68
C07H021/04
C12P019/34
C12N009/22
C12P021/02
C12N001/21
C12N015/74
US Classification:

435/006000, 435/069100, 435/091200, 435/199000, 435/252310, 435/320100, 536/023200
Abstract:

The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polymerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.

Resumes

Professor Of Chemistry

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Location:

New York, NY

Industry:

Higher Education

Work:

City College of New York
Professor of Chemistry
Harvard University Jul 2002 - Jun 2012
Associate Professor
Rockefeller University 1996 - 2002
Research Associate

Education:

Yale University 1987 - 1994
Doctorates, Doctor of Philosophy, Philosophy, Molecular Biophysics