Daniel J Ramon

US Patent:

20050176023, Aug 11, 2005

Filed:

Jul 30, 2004

Appl. No.:

10/909278

Inventors:

Daniel Ramon - Brown Deer WI, US
Lu Wang - Shorewood WI, US

International Classification:

C12Q001/68
C07H021/04

US Classification:

435006000, 536024300

Abstract:

The present invention provides probes for target nucleic acid sequences and methods for using the probes to detect the presence or absence of a desired target nucleic acid sequence. The present probes contain two or more probing regions. The probes also contain multiple self-complementary sequences that hybridize to one another in the absence of one or more of the target sequences. The probes can also optionally contain one or more arm sequences, spacer sequences or labels as desired.

US Patent:

20040014101, Jan 22, 2004

Filed:

May 5, 2003

Appl. No.:

10/429912

Inventors:

Xiangjun Liu - Menomonee Falls WI, US
Lu Wang - Shorewood WI, US
Daniel Ramon - Brown Deer WI, US

Assignee:

Pel-Freez Clinical Systems, Inc.

International Classification:

C12Q001/68

US Classification:

435/006000

Abstract:

The present invention provides methods for analyzing polymorphic nucleic acids using duplex separation and/or identification techniques. In the present methods one of the nucleic acids has a sequence which includes universal bases that correspond in position to one or more of the polymorphic positions of the polymorphic nucleic acid. The nucleic acid including the universal bases can either represent the polymorphic nucleic acid and hybridize with a reference strand, or act as a reference strand and hybridize with the polymorphic nucleic acid directly, to form a duplex. Separation and/or identification of the duplex from other duplexes and materials can then be performed.

Author:

Daniel Ramon

ISBN #:

8476603193