Gwynedd A. Benders - Portland OR, US John I. Glass - Germantown MD, US Carole Lartigue - Des Arenes Bayonne, FR Sanjay Vashee - Boyds MD, US Mikkel A. Algire - Jessup MD, US Hamilton O. Smith - San Diego CA, US Charles E. Merryman - Sykesville MD, US Vladimir N. Noskov - Montgomery Village MD, US Ray-Yuan Chuang - Rockville MD, US Daniel G. Gibson - Crofton MD, US J. Craig Venter - La Jolla CA, US
Compositions and methods are disclosed herein for cloning a donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.
Gwynedd A. Benders - Portland OR, US John I. Glass - Germantown MD, US Clyde A. Hutchison - La Jolla CA, US Carole Lartigue - Des Arenes Bayonne, FR Sanjay Vashee - Boyds MD, US Mikkel A. Algire - Jessup MD, US Hamilton O. Smith - San Diego CA, US Charles E. Merryman - Sykesville MD, US Vladimir N. Noskov - Montgomery Village MD, US Ray-Yuan Chuang - Rockville MD, US Daniel G. Gibson - Crofton MD, US J. Craig Venter - La Jolla CA, US
Assignee:
Synthetic Genomics, Inc. - La Jolla CA
International Classification:
C12N 15/87 C12N 1/21
US Classification:
435455, 435471, 435468, 4352523
Abstract:
Compositions and methods are disclosed herein for cloning a synthetic or a semi-synthetic donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.
- Redwood City CA, US Daniel Valentine Gibson - Irvine CA, US Kenneth Alan Moss - Redwood City CA, US
International Classification:
A63F 13/795 A63F 13/61
Abstract:
Embodiments of the systems and methods disclosed herein provide a sponsor matching system in which players and sponsors can be matched. Upon a match based at least in part on stored sponsorship criteria and/or player preferences, a first sponsor can select a set of players to receive permission to select an advertisement associated with the first sponsor. Once a first player of the selected players selects an advertisement and an advertisement placement location associated with the first sponsor, the sponsor matching system can generate game rendering instructions for a first player system associated with the first player.
- Redwood City CA, US Daniel Valentine Gibson - Irvine CA, US Kenneth Alan Moss - Redwood City CA, US
International Classification:
A63F 13/795 A63F 13/61
Abstract:
Embodiments of the systems and methods disclosed herein provide a sponsor matching system in which players and sponsors can be matched. Upon a match based at least in part on stored sponsorship criteria and/or player preferences, a first sponsor can select a set of players to receive permission to select an advertisement associated with the first sponsor. Once a first player of the selected players selects an advertisement and an advertisement placement location associated with the first sponsor, the sponsor matching system can generate game rendering instructions for a first player system associated with the first player.
- La Jolla CA, US Jun Urano - Irvine CA, US Nicky C. Caiazza - Rancho Santa Fe CA, US Daniel G. Gibson - Carlsbad CA, US
International Classification:
C12P 19/34 C12N 15/10 C12Q 1/6844
Abstract:
The present invention discloses methods for assembling a nucleic acid molecule from a set of overlapping oligonucleotides. The method involves contacting a set of overlapping oligonucleotides with a DNA polymerase, a mixture of dNTPs, and a crowding agent to form an assembly mixture. In one embodiment the crowding agent is polyethylene glycol (PEG). The presence of the crowding agent facilitates the nucleic acid assembly process of the invention. The assembly mixture is then subjected to multiple cycles, each cycle comprising an annealing phase, an extension phase, and a denaturation phase, and the desired nucleic acid molecule is thereby assembled. In some embodiments one or more of the phases are time varied.
- La Jolla CA, US Jun Urano - Irvine CA, US Nicky C. Caiazza - Rancho Santa Fe CA, US Daniel G. Gibson - Carlsbad CA, US
Assignee:
Synthetic Genomics, Inc. - La Jolla CA
International Classification:
C12P 19/34
US Classification:
435 912
Abstract:
The present invention discloses methods for assembling a nucleic acid molecule from a set of overlapping oligonucleotides. The method involves contacting a set of overlapping oligonucleotides with a DNA polymerase, a mixture of dNTPs, and a crowding agent to form an assembly mixture. In one embodiment the crowding agent is polyethylene glycol (PEG). The presence of the crowding agent facilitates the nucleic acid assembly process of the invention. The assembly mixture is then subjected to multiple cycles, each cycle comprising an annealing phase, an extension phase, and a denaturation phase, and the desired nucleic acid molecule is thereby assembled. In some embodiments one or more of the phases are time varied.
Name / Title
Company / Classification
Phones & Addresses
Daniel Gibson Managing
Boobysworld, LLC Entertainment Services
1900 Ave Of The Stars, Los Angeles, CA 90067 2804 Gtwy Oaks Dr, Sacramento, CA 95833
Daniel Gibson Managing
Fitfam Sa LLC
Daniel D. Gibson
DAN GIBSON CONSTRUCTION LLC
Daniel Gibson
FISHERS OF MEN U.S.A
Daniel Gibson Manager
BOYS AND GIRLS CLUB OF ANAHEIM, INC Youth Center
311 E Broadway, Anaheim, CA 92805 PO Box 350, Anaheim, CA 92815 714 491-3617, 714 491-3950
Capital Region Medical ClinicCapital Region Health Branch West 3308 W Edgewood Dr STE B, Jefferson City, MO 65109 573 893-7848 (phone), 573 893-1984 (fax)
Capital Region Medical ClinicCapital Region Physicians Urgent Care 220 Madison St, Jefferson City, MO 65101 573 632-4900 (phone), 573 644-6650 (fax)
Education:
Medical School Oklahoma State University Center for Health Sciences College of Osteopathic Medicine Graduated: 1999
Procedures:
Allergen Immunotherapy Electrocardiogram (EKG or ECG) Vaccine Administration Wound Care
Dr. Gibson graduated from the Oklahoma State University Center for Health Sciences College of Osteopathic Medicine in 1999. He works in Jefferson City, MO and 1 other location and specializes in Family Medicine and Urgent Care Medicine. Dr. Gibson is affiliated with Capital Region Medical Center.