Dalmacio A Agdeppa

US Patent:

59324317, Aug 3, 1999

Filed:

Apr 16, 1997

Appl. No.:

8/840782

Inventors:

Gregg T. Williams - Villa Park IL
William R. Groskopf - Libertyville IL
Harold N. Baker - Libertyville IL
Dalmacio A. Agdeppa - Morton Grove IL

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

G01N 3353

US Classification:

435 793

Abstract:

A method and kit for measurement of a steroid by means of a competitive immunoassay, preferably a competitive enzyme immunoassay. The method and kit involve the use of a steroid analogue conjugated to a label. The steroids that are amenable to detection by the method and kit of the present invention include estradiol and progesterone. The method comprises the steps of: a. incubating a mixture of a test sample suspected of containing a given steroid, a solid phase coupled to an antibody specific for that steroid, and a conjugate of an analogue of that steroid to form steroid/antibody complexes and conjugate/antibody complexes on said solid phase; b. separating said solid phase from said mixture; c. measuring the amount of label present in said mixture or in said solid phase; and d. determining the amount of steroid in said sample from the amount of label.

US Patent:

62011413, Mar 13, 2001

Filed:

Jun 18, 1999

Appl. No.:

9/336884

Inventors:

Gregg T. Williams - Villa Park IL
William R. Groskopf - Libertyville IL
Harold N. Baker - Libertyville IL
Dalmacio A. Agdeppa - Morton Grove IL

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

C07J 700

US Classification:

552599

Abstract:

A method and kit for measurement of a steroid by means of a competitive immunoassay, preferably a competitive enzyme immunoassay. The method and kit involve the use of a steroid analogue conjugated to a label. The steroids that are amenable to detection by the method and kit of the present invention include estradiol and progesterone. The method comprises the steps of: a. incubating a mixture of a test sample suspected of containing a given steroid, a solid phase coupled to an antibody specific for that steroid, and a conjugate of an analogue of that steroid to form steroid/antibody complexes and conjugate/antibody complexes on said solid phase; b. separating said solid phase from said mixture; c. measuring the amount of label present in said mixture or in said solid phase; and d. determining the amount of steroid in said sample from the amount of label.

US Patent:

56630549, Sep 2, 1997

Filed:

Mar 3, 1995

Appl. No.:

8/398226

Inventors:

Gregg T. Williams - Villa Park IL
William R. Groskopf - Libertyville IL
Harold N. Baker - Libertyville IL
Dalmacio A. Agdeppa - Morton Grove IL

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

G01N 3353
G01N 33542
G01N 33537
G01N 33543

US Classification:

435 793

Abstract:

A method and kit for measurement of asteroid by means of a competitive immunoassay, preferably a competitive enzyme immunoassay. The method and kit involve the use of asteroid analogue conjugated to a label. The steroids that are amenable to detection by the method and kit of the present invention include estradiol and progesterone. The method comprises the steps of: a. incubating a mixture of a test sample suspected of containing a given steroid, a solid phase coupled to an antibody specific for that steroid, and a conjugate of an analogue of that steroid to form steroid/antibody complexes and conjugate/antibody complexes on said solid phase; b. separating said solid phase from said mixture; c. measuring the amount of label present in said mixture or in said solid phase; and d. determining the amount of steroid in said sample from the amount of label.