Christopher J Fisher

US Patent:

20090305334, Dec 10, 2009

Filed:

Aug 12, 2009

Appl. No.:

12/539947

Inventors:

Anthony W. Dallmier - Concord OH, US
Christopher W. Fisher - Mentor-on-the-Lake OH, US
Timothy J. Millett - Mentor OH, US

International Classification:

C12Q 1/02
C12N 13/00

US Classification:

435 29, 4351739

Abstract:

A biological indicator and method of making same. The biological indicator includes a carrier having a recess formed therein in order to restrict movement of an inoculum deposited onto the carrier. The inoculum includes microorganisms (e.g., bacterial spores) suspended in a suspension medium. The microorganisms are prepared by removing extraneous material and subjecting the microorganisms to sonication to break up agglomerations. The suspension medium includes a wetting agent to reduce surface tension, thereby facilitating flow of the suspension medium to prevent stacking of microorganisms on the surface of the carrier, and to allow the inoculum to more evenly “plate out” on carrier surfaces. The carrier, with inoculum deposited thereon, is enclosed in an envelope made of a material permeable to a vaporous deactivating agent (e.g., vaporized hydrogen peroxide, ozone, chlorine dioxide, ethylene oxide, etc.), thereby facilitating exposure to the vaporous deactivating agent.

US Patent:

20080206801, Aug 28, 2008

Filed:

Feb 27, 2007

Appl. No.:

11/679208

Inventors:

Anthony W. Dallmier - Concord OH, US
Christopher W. Fisher - Mentor-on-the-Lake OH, US
Timothy J. Millett - Mentor OH, US

International Classification:

C12Q 1/02

US Classification:

435 29

Abstract:

A biological indicator and method of making same. The biological indicator includes a carrier having a recess formed therein in order to restrict movement of an inoculum deposited onto the carrier. The inoculum includes microorganisms (e.g., bacterial spores) suspended in a suspension medium. The microorganisms are prepared by removing extraneous material and subjecting the microorganisms to sonication to break up agglomerations. The suspension medium includes a wetting agent to reduce surface tension, thereby facilitating flow of the suspension medium to prevent stacking of microorganisms on the surface of the carrier, and to allow the inoculum to more evenly “plate out” on carrier surfaces. The carrier, with inoculum deposited thereon, is enclosed in an envelope made of a material permeable to a vaporous deactivating agent (e.g., vaporized hydrogen peroxide, ozone, chlorine dioxide, ethylene oxide, etc.), thereby facilitating exposure to the vaporous deactivating agent.

Author:

Christopher T. Fisher

ISBN #:

0387327614