Christina Chang Chang

US Patent:

20220315988, Oct 6, 2022

Filed:

Jun 2, 2022

Appl. No.:

17/830897

Inventors:

- Franklin Lakes NJ, US
Eleen Shum - San Jose CA, US
Christina Chang - San Jose CA, US

International Classification:

C12Q 1/6806
C12Q 1/6851
C12Q 1/6876

Abstract:

Disclosed herein include systems, methods, compositions, and kits for PCR normalization. In some embodiments, after barcoding copies of a higher abundance target (e.g., a cDNA species), the barcoded copies are amplified using a pair of forward primers comprising one or more mismatches and a reverse primer. The amplified copies can be further linearly amplified using a forward primer comprising the sequence of one of the pair of forward primers, and a reverse primer.

US Patent:

20230125113, Apr 27, 2023

Filed:

Aug 22, 2022

Appl. No.:

17/821449

Inventors:

- Franklin Lakes NJ, US
Christina Chang - Menlo Park CA, US
Eleen Shum - Menlo Park CA, US
Olaf Zoellner - Menlo Park CA, US
James Ghadiali - Menlo Park CA, US
Gretchen Yinbon Lam - Menlo Park CA, US
Eric Jensen - Franklin Lakes NJ, US
Jurg Rohrer - Franklin Lakes NJ, US
Brent Gaylord - Franklin Lakes NJ, US
Jody Martin - Franklin Lakes NJ, US
Jason G. Vidal - Franklin Lakes NJ, US

International Classification:

G01N 33/566
G16B 99/00
G01N 33/50
G01N 33/543
G01N 33/68
C12Q 1/6804
G16Z 99/00
C12N 15/10
C12Q 1/6806
C12Q 1/6823
C12Q 1/6869
C12Q 1/68

Abstract:

Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.

US Patent:

20230057339, Feb 23, 2023

Filed:

Aug 25, 2022

Appl. No.:

17/895633

Inventors:

- Palo Alto CA, US
Hei Mun Christina Fan - Palo Alto CA, US
Anaram Shahravan - San Jose CA, US
Christina Chang - Palo Alto CA, US
Jay Levine - San Leandro CA, US

International Classification:

C12Q 1/6816
C12Q 1/6853

Abstract:

Systems, methods, and compositions for generating a high-resolution spatial map of a distribution of targets of a sample are described. Processes for generating the spatial map can include: receiving the sample at a substrate having a distribution of functionalized particles, each having a stochastic barcode sequence paired with a position on the substrate; promoting interactions between the distribution of targets of the sample and the distribution of functionalized particles upon transmitting heat to a surface of the substrate opposite the distribution of functionalized particles; applying a set of reactions to the sample at the substrate, obtaining a set of sequences of a population of molecules generated from the set of reactions, the set of sequences associated with the distribution of targets labeled using the stochastic barcode sequences of the distribution of functionalized particles, and returning a set of positions of the distribution of targets upon processing the set of sequences.

US Patent:

20200248263, Aug 6, 2020

Filed:

Jan 21, 2020

Appl. No.:

16/748691

Inventors:

- Franklin Lakes NJ, US
Eleen Shum - San Carlos CA, US
Christina Chang - San Jose CA, US

International Classification:

C12Q 1/6883
G01N 33/53

Abstract:

Disclosed herein include systems, methods, compositions, and kits for quantitative analysis of a plurality of cellular component targets of cells of interest. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) is associated with one or more detectable moieties (e.g., luminescent moieties, fluorescent moieties, a phosphorescent moieties). In some embodiments, the presence of the detectable moiety in the binding reagent oligonucleotide enables the cellular component-binding reagent to be employed for both fluorescence analysis (e.g., cell sorting) and sequence analysis (e.g., protein expression profiling).

US Patent:

20200232032, Jul 23, 2020

Filed:

Jan 21, 2020

Appl. No.:

16/747737

Inventors:

- San Jose CA, US
Katherine Lazaruk - San Jose CA, US
Dennis E. Prosen - San Jose CA, US
Devon Jensen - San Jose CA, US
David Rosenfeld - San Jose CA, US
Janice H. Lai - San Jose CA, US
James Ghadiali - San Jose CA, US
Christina Chang - San Jose CA, US

International Classification:

C12Q 1/6876
C12Q 1/6806
C12Q 1/6853

Abstract:

Disclosed herein include systems, methods, compositions, and kits for determining protein expression and gene expression simultaneously and for sample indexing. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) comprises one or more of a unique molecular label sequence, a primer adapter, antibody-specific barcode sequence, an alignment sequence, and/or a poly(A) sequence. In some embodiments, the oligonucleotide is associated with the cellular component-binding reagent via a linker (e.g., 5AmMC12).

US Patent:

20200224247, Jul 16, 2020

Filed:

Jan 15, 2020

Appl. No.:

16/743898

Inventors:

- Franklin Lakes NJ, US
Eleen Shum - San Jose CA, US
Christina Chang - San Jose CA, US

International Classification:

C12Q 1/6806
C12Q 1/6851
C12Q 1/6876

Abstract:

Disclosed herein include systems, methods, compositions, and kits for PCR normalization. In some embodiments, after barcoding copies of a higher abundance target (e.g., a cDNA species), the barcoded copies are amplified using a pair of forward primers comprising one or more mismatches and a reverse primer. The amplified copies can be further linearly amplified using a forward primer comprising the sequence of one of the pair of forward primers, and a reverse primer.

US Patent:

20200040379, Feb 6, 2020

Filed:

Jul 29, 2019

Appl. No.:

16/525054

Inventors:

- Menlo Park CA, US
Eleen Shum - Menlo Park CA, US
Christina Chang - Menlo Park CA, US
Christina Fan - Menlo Park CA, US

International Classification:

C12Q 1/6806
C12Q 1/6813

Abstract:

Disclosed herein include methods, compositions, and kits suitable for use in performing single nuclei capture and barcoding in a single step. In some embodiments, the method comprises isolating nuclei using a nuclei-isolation composition. The nuclei-isolation composition can comprise a nuclei-binding reagent capable of specifically binding to one or more components of a nucleus. The method can include barcoding targets in the nuclei using barcodes to generate barcoded targets for sequencing analysis.

Address:

210 Sebastian Dr, Millbrae, CA 94030

Phone:

650 548-1005

VIN:

WDDEJ71X98A018483

Make:

MERCEDES-BENZ

Model:

CL-CLASS

Year:

2008