Skills:
Molecular biology and microbiology techniques: Sub-cloning and maintenance human normal or cancer cells, and bacteria strains, ligation, electroporation, transfection with bacteriophage, and transduction; extraction cell components such as nucleus and cytoplasm, DNA, RNA and proteins. Genetic techniques: Gene engineering techniques, DNA and RNA isolation and purification, reverse transcription, polymerase chain reaction (PCR), real-time PCR (RT-PCR), array-based SYBR Green RT-PCR, gene mutation and deletion, and so on. Proteomic techniques: Antibody microarray, glycan-detection antibody microarray, two-dimensional gel electrophoresis (2D), 2D difference gel electrophoresis (2D-DIGE), Western blot, immunoprecipitation, immunohistochemical (IHC), immunocytochemistry (ICC), protein digestion, and protein identification by mass spectrometry (LC-MS, MALDI-TOF). Biostatistics: SAS, SPSS, R, Epi data, FoxBase+, Sigmaplot, and so on. Computer skills: Microsoft Office (Word, Excel, PowerPoint, Access) and protein gel analysis softwares (DeCyder, ImageMaster, ImageQuant, Quantity One, and etc.).