Bruce C Currie

Author:

Bruce L. Currie

ISBN #:

0683022601

US Patent:

39446590, Mar 16, 1976

Filed:

Dec 13, 1972

Appl. No.:

5/314750

Inventors:

Karl Folkers - Austin TX
Karl Nils Gunnar Johansson - Austin TX
Bruce L. Currie - Austin TX

International Classification:

A61k 2704
A61k 1708

US Classification:

424 1

Abstract:

A method for producing the Folliculotropin Releasing Hormone (FRH) biosynthetically is provided by utilizing, as key starting materials, fresh hypothalamic tissue, a buffered incubation medium, an ATP synthesizing system, radioactively labeled amino acids and a mixture of 21 naturally occuring amino acids. Biosynthesis of the FRH is accomplished by incubation of the hypothalamic tissue for a short period of time in the presence of all the reagents necessary to promote the biosynthetic system to produce the Folliculotropin Releasing Hormone (FRH) of the hypothalamus gland of mammals. Examples of methods to isolate the FRH free of the luteotropin releasing hormone (LRH) are provided.

US Patent:

39534162, Apr 27, 1976

Filed:

Jan 14, 1974

Appl. No.:

5/432903

Inventors:

Karl Folkers - Austin TX
Bruce L. Currie - Austin TX
Hans Sievertsson - 191 56 Sollentuna, SW
Conny Bogentoft - 19500 Marsta, SW

International Classification:

C07C10352
C07G 700

US Classification:

2601125LH

Abstract:

A synthetic decapeptide, L-pglutamyl-L-histidyl-L-tryptophanyl-L-seryl-L-tyrosyl-glycyl-L-leucyl-L- arginyl-L-prolyl-glycinamide which has the hormonal activities of the luteinizing hormone releasing hormone (LRH) of the hypothalamus gland of mammals is produced by utilizing as the key starting materials, the amino acids, glutamic acid or pyroglutamic acid, histidine, tryptophan, serine, tyrosine, glycine, leucine, arginine, and proline. Synthesis of the decapeptide is accomplished by coupling, in appropriate combinations of appropriate protected forms of the amino acids, and finally deprotecting to yield the amide, L-pglutamyl-L-histidyl-L-tryptophanyl-L-seryl-L-tyrosyl-glycyl-L-leucyl-L- arginyl-L-prolyl-glycinamide.