Brian M Turczyk from 36 Carlisle Rd, Graniteville, MA 01886, age 47, Phone: 978 577-6087

Resumes

Staff Scientist At Wyss Institute For Biologically Inspired Engineering

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Position:

Staff Scientist at Wyss Institute for Biologically Inspired Engineering

Location:

Greater Boston Area

Industry:

Biotechnology

Work:

Wyss Institute for Biologically Inspired Engineering - Greater Boston Area since Mar 2012
Staff Scientist
Modular Genetics, Inc. Feb 2005 - Apr 2011
Senior Scientist

Education:

Boston University School of Medicine 1998 - 2005
Ph.D., Pharmacology, Molecular Biology University of New Hampshire 1994 - 1998
Biochemistry, Molecular biology, microbiology

Us Patents

System For Manipulating Nucleic Acids
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US Patent:

8129119, Mar 6, 2012
Filed:

May 4, 2006
Appl. No.:

11/417754
Inventors:

Kevin Jarrell - Lincoln MA, US
Brian Turczyk - Peabody MA, US
Assignee:

Modular Genetics, Inc. - Cambridge MA
International Classification:

C12Q 1/68
C12P 19/34
US Classification:

435 612, 435 912, 435 914, 435 691
Abstract:

The present invention provides an improved system for linking nucleic acids to one another. In particular, the present invention provides techniques for producing DNA product molecules that may be easily and directly ligated to recipient molecules. The product molecules need not be cleaved with restriction enzymes in order to undergo such ligation. In preferred embodiments of the invention, the DNA product molecules are produced through iterative DNA synthesis reactions, so that the product molecules are amplified products. The invention further provides methods for directed ligation of product molecules (i. e. , for selective ligation of certain molecules within a collection of molecules), and also for methods of exon shuffling, in which multiple different product molecules are produced in a single ligation reaction. Preferred embodiments of the invention involve ligation of product molecules encoding functional protein domains, particularly domains naturally found in conserved gene families. The inventive DNA manipulation system is readily integrated with other nucleic acid manipulation systems, such as ribozyme-mediated systems, and also is susceptible to automation.

System For Manipulating Nucleic Acids
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US Patent:

20040005673, Jan 8, 2004
Filed:

Oct 15, 2002
Appl. No.:

10/272351
Inventors:

Kevin Jarrell - Lincoln MA, US
Brian Turczyk - Peabody MA, US
International Classification:

C12P019/34
C12N009/00
C07H021/04
C12P021/02
C12N005/06
US Classification:

435/091200, 435/069100, 435/320100, 435/325000, 435/183000, 536/023200
Abstract:

The present invention provides an improved system for linking nucleic acids to one another. In particular, the present invention provides techniques for producing DNA product molecules that may be easily and directly ligated to recipient molecules. The product molecules need not be cleaved with restriction enzymes in order to undergo such ligation. In preferred embodiments of the invention, the DNA product molecules are produced through iterative DNA synthesis reactions, so that the product molecules are amplified products. The invention further provides methods for directed ligation of product molecules (i.e., for selective ligation of certain molecules within a collection of molecules), and also for methods of exon shuffling, in which multiple different product molecules are produced in a single ligation reaction. Preferred embodiments of the invention involve ligation of product molecules encoding functional protein domains, particularly domains naturally found in conserved gene families. The inventive DNA manipulation system is readily integrated with other nucleic acid manipulation systems, such as ribozyme-mediated systems, and also is susceptible to automation.

Modular Vector Systems
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US Patent:

20040161752, Aug 19, 2004
Filed:

Mar 5, 2003
Appl. No.:

10/383135
Inventors:

Kevin Jarrell - Lincoln MA, US
William Donahue - Quincy MA, US
Brian Turczyk - Peabody MA, US
International Classification:

C12Q001/68
C07H021/04
C12P019/34
US Classification:

435/006000, 435/091200, 536/023200
Abstract:

The present invention provides improved techniques and reagents for producing nucleic acid molecules. In certain preferred embodiments, the nucleic acid molecules are modular vectors. In certain preferred embodiments, the nucleic acid molecules are produced in polymerase chain reactions employing terminator primer residues.

Modular Vector Systems
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US Patent:

20090176281, Jul 9, 2009
Filed:

Oct 14, 2008
Appl. No.:

12/251145
Inventors:

Kevin A. Jarrell - Lincoln MA, US
William F. Donahue - Quincy MA, US
Brian M. Turczyk - Peabody MA, US
International Classification:

C12P 19/34
C12N 15/11
US Classification:

435 912, 4353201
Abstract:

The present invention provides improved techniques and reagents for producing nucleic acid molecules. In certain preferred embodiments, the nucleic acid molecules are modular vectors. In certain preferred embodiments, the nucleic acid molecules are produced in polymerase chain reactions employing terminator primer residues.

Processive Template Independent Dna Polymerase Variants
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US Patent:

20190360013, Nov 28, 2019
Filed:

Dec 4, 2017
Appl. No.:

16/465816
Inventors:

- Cambridge MA, US
Brian M. TURCZYK - Westford MA, US
Daniel Jordan WIEGAND - Somerville MA, US
George M. CHURCH - Brookline MA, US
Assignee:

President and Fellows of Harvard College - Cambridge MA
International Classification:

C12P 19/34
C12N 9/12
Abstract:

An enzymatic method of making a polynucleotide is provided. The method includes combining a selected nucleotide triphosphate, one or more cations, a template-independent polymerase, and an associated processivity factor in an aqueous reaction medium including a target substrate comprising an initiator sequence and having a 3′ terminal nucleotide attached to a single stranded portion, such that the template-independent polymerase and the associated processivity factor interact with the target substrate under conditions which covalently add one or more of the selected nucleotide triphosphate to the 3′ terminal nucleotide. Also provided are mutant template-independent polymerases having a processivity factor attached thereto.

Methods Of Generating Libraries Of Nucleic Acid Sequences For Detection Via Fluorescent In Situ Sequ
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US Patent:

20190330617, Oct 31, 2019
Filed:

Jul 1, 2019
Appl. No.:

16/458268
Inventors:

George M. Church - Brookline MA, US
Evan R. Daugharthy - Cambridge MA, US
Richard C. Terry - Carlisle MA, US
Benjamin W. Pruitt - Cambridge MA, US
Brian M. Turczyk - Westford MA, US
Assignee:

President and Fellows of Harvard College - Cambridge MA
International Classification:

C12N 15/10
C12Q 1/6869
C12Q 1/6844
C12Q 1/6832
C12Q 1/6874
C12Q 1/6816
C12Q 1/6806
Abstract:

The present disclosure provides a number of targeted nucleic acid FISSEQ library construction methods. Targeted FISSEQ can exhibit several benefits, such as enhanced sensitivity and/or shorter assay time in the detection, identification, quantification, and/or determining the nucleotide sequence of the target species, relative to “random” or “whole-omic” detection via FISSEQ.

Methods Of Generating Libraries Of Nucleic Acid Sequences For Detection Via Flourescent In Situ Sequencing
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US Patent:

20190177718, Jun 13, 2019
Filed:

Feb 26, 2019
Appl. No.:

16/285292
Inventors:

- Cambridge MA, US
Evan R. Daugharthy - Cambridge MA, US
Richard C. Terry - Carlisle MA, US
Benjamin W. Pruitt - Cambridge MA, US
Brian M. Turczyk - Westford MA, US
International Classification:

C12N 15/10
C12Q 1/6806
C12Q 1/6832
C12Q 1/6874
C12Q 1/6816
Abstract:

The present disclosure provides a number of targeted nucleic acid FISSEQ library construction methods. Targeted FISSEQ can exhibit several benefits, such as enhanced sensitivity and/or shorter assay time in the detection, identification, quantification, and/or determining the nucleotide sequence of the target species, relative to “random” or “whole-omic” detection via FISSEQ.