Brian A Erickson

US Patent:

20230132372, Apr 27, 2023

Filed:

Jul 28, 2021

Appl. No.:

17/387888

Inventors:

- Cambridge MA, US
- Boston MA, US
Steven P. Gygi - Cambridge MA, US
Gregory H. Bird - Pelham NH, US
Loren D. Walensky - Newton MA, US
Martin Helmut Wuhr - Cambridge MA, US
Brian K. Erickson - Cambridge MA, US

Assignee:

President and Fellows of Harvard College - Cambridge MA
Dana-Farber Cancer Institute, Inc. - Boston MA

International Classification:

G01N 33/58
C07D 207/46
C07C 237/22
C07K 1/13
C07K 5/02
G01N 33/68

Abstract:

In some embodiments, a mass spectrometry tag may comprise a linker region, a mass balance region, and a reporter region. The mass spectrometry tag may be configured to fragment in a mass spectrometer via an energy dependent process to produce multiple reporter molecules. For example, the reporter region of the tag may be configured to produce at least two reporter molecules via fragmentation. In some embodiments, one or more regions of the tag may comprise at least one heavy isotope. In some such embodiments, the ability to fragment into multiple reporter molecules as well as the placement and/or number of heavy isotope(s) allows the mass spectrometry tag to be distinguished from other similar mass spectrometry tags. In some such embodiments, the ability to distinguish between tags having the same or substantially similar total mass to charge ratio and reporter region mass may allow the system to have a greater multiplexing capacity than conventional systems.

US Patent:

20200174007, Jun 4, 2020

Filed:

Nov 22, 2019

Appl. No.:

16/692762

Inventors:

- Cambridge MA, US
- Boston MA, US
Steven P. Gygi - Foxborough MA, US
Gregory H. Bird - Pelham NH, US
Loren D. Walensky - Newton MA, US
Martin Helmut Wuhr - Malden MA, US
Brian K. Erickson - Brookline MA, US

Assignee:

President and Fellows of Harvard College - Cambridge MA
Dana-Farber Cancer Institute, Inc. - Boston MA

International Classification:

G01N 33/58
C07D 207/46
C07C 237/22
C07K 1/13
C07K 5/02
G01N 33/68

Abstract:

In some embodiments, a mass spectrometry tag may comprise a linker region, a mass balance region, and a reporter region. The mass spectrometry tag may be configured to fragment in a mass spectrometer via an energy dependent process to produce multiple reporter molecules. For example, the reporter region of the tag may be configured to produce at least two reporter molecules via fragmentation. In some embodiments, one or more regions of the tag may comprise at least one heavy isotope. In some such embodiments, the ability to fragment into multiple reporter molecules as well as the placement and/or number of heavy isotope(s) allows the mass spectrometry tag to be distinguished from other similar mass spectrometry tags. In some such embodiments, the ability to distinguish between tags having the same or substantially similar total mass to charge ratio and reporter region mass may allow the system to have a greater multiplexing capacity than conventional systems.

US Patent:

20190310264, Oct 10, 2019

Filed:

Jun 1, 2017

Appl. No.:

16/306686

Inventors:

- Cambridge MA, US
Brian K. Erickson - Brookline MA, US
Christopher Michael Rose - Fremont CA, US
Steven P. Gygi - Foxborough MA, US

Assignee:

President and Fellows of Harvard College - Cambridge MA

International Classification:

G01N 33/68

Abstract:

Described herein is a kit of materials prepared for assays that involve determining relative abundance and/or absolute abundance of various targeted peptides. The kit may comprise trigger versions of target peptides with masses offset from the respective target peptides by predetermined and known amounts. The trigger peptides may be present in amounts that may be readily detected via a mass spectrometry analysis. When mixed with samples that are analyzed, detection of the trigger peptides indicates where in the mass-spectrometer output the target peptide may be found. The kit may include a predetermined amount of synthetic versions of one or more of the target peptides. A measured relative abundance of this synthetic peptide relative to that of the target peptides yields an absolute quantitative value of the target peptide. Also disclosed is a method of preparing a plurality of samples to be submitted for mass spectrometer analysis in parallel.

US Patent:

20180209985, Jul 26, 2018

Filed:

Jun 3, 2016

Appl. No.:

15/578382

Inventors:

- Cambridge MA, US
- Boston MA, US
Steven P. Gygi - Foxborough MA, US
Gregory H. Bird - Pelham NH, US
Loren D. Walensky - Newton MA, US
Martin Helmut Wuhr - Malden MA, US
Brian K. Erickson - Brookline MA, US

Assignee:

President and Fellows of Harvard College - Cambridge MA
Dana-Faber Cancer Institute, Inc. - Boston MA

International Classification:

G01N 33/58
C07D 207/46

Abstract:

In some embodiments, a mass spectrometry tag may comprise a linker region, a mass balance region, and a reporter region. The mass spectrometry tag may be configured to fragment in a mass spectrometer via an energy dependent process to produce multiple reporter molecules. For example, the reporter region of the tag may be configured to produce at least two reporter molecules via fragmentation. In some embodiments, one or more regions of the tag may comprise at least one heavy isotope. In some such embodiments, the ability to fragment into multiple reporter molecules as well as the placement and/or number of heavy isotope(s) allows the mass spectrometry tag to be distinguished from other similar mass spectrometry tags. In some such embodiments, the ability to distinguish between tags having the same or substantially similar total mass to charge ratio and reporter region mass may allow the system to have a greater multiplexing capacity than conventional systems.