Nucleic acid sequences are provided that are useful as amplification primers, hybridization probes, and as a portion of molecular beacon probes for amplifying and detecting polymorphisms of the 2 adrenergic receptor gene, compositions and kits incorporating the same, and methods employing the same.
Nucleic acid sequences are provided that are useful as amplification primers and hybridization probes for amplifying and detecting target sequence from the β2 adrenergic receptor gene. The primers and probes can be employed in amplification based methods for detecting the presence of the target sequence in a test sample.
Jeffrey B. Huff - Park Ridge IL Barbara T. Merchant - Wilmette IL Carolyn R. Mullen - Libertyville IL Seshagiri R. Tata Venkata - Chicago IL
Assignee:
Abbott Laboratories - Abbott Park IL
International Classification:
C07H 1704 C07D31102
US Classification:
536 41
Abstract:
Fluorescent enzyme substrates are provided. The substrates are derivatives of coumarin having an enzyme cleavable group at position 7 and a hydrophilic amide group at position 4. The compounds are highly water soluble, stable at ambient and reduced temperatures and fluoresce upon cleavage by an enzyme.