The United States of America as represented by the Secretary of Agriculture - Washington DC
International Classification:
C12P007/18
US Classification:
435158, 435137, 435189, 435105, 435170
Abstract:
Mannitol is produced in a highly efficient fermentative method using NRRL B-30560, or in a biochemical method using mannitol dehydrogenase isolated from this strain. Fructose serves as the primary carbon substrate in both the fermentative and biochemical conversions, but important secondary carbon sources include glucose, maltose, mannose and galactose. Mannitol is useful in the food, pharmaceutical, and medicine industries as a sweet-tasting bodying and texturing agent.
The United States of America as represented by the Secretary of Agriculture - Washington DC
International Classification:
C12N 9/04
US Classification:
435190, 4352529, 424 941
Abstract:
Mannitol is produced in a highly efficient fermentative method using NRRL B-30560, or in a biochemical method using mannitol dehydrogenase isolated from this strain. Fructose serves as the primary carbon substrate in both the fermentative and biochemical conversions, but important secondary carbon sources include glucose, maltose, mannose and galactose. Mannitol is useful in the food, pharmaceutical, and medicine industries as a sweet-tasting bodying and texturing agent.
Methods For Production Of Xylitol In Microorganisms
Paul Taylor - Arlington Heights IL, US Ian Fotheringham - Schaumburg IL, US Nathan Wymer - Peoria IL, US Badal Saha - Peoria IL, US David Demirjian - Hinsdale IL, US Yoshikiyo Sakaibara - Peoria IL, US Francis Michael Racine - Peoria IL, US
Assignee:
Biotechnology Research Development Corporation - Peoria IL
International Classification:
C12P 7/60 C12N 1/21
US Classification:
435138, 43525233, 4352523
Abstract:
The invention provides biosynthetic routes to xylitol production that do not require pure -xylose for synthesis and that can utilize inexpensive substrates such as hemicellulose hydrolysates.
Method For Microbial Production Of Xylitol From Arabinose
Recombinant microorganisms are useful for producing xylitol by fermentation of arabinose. The recombinant microorganisms are produced by transformation of host microorganisms with heterologous polynucleotide sequences coding for each of L-xylulose reductase, D-tagatose 3-epimerase, and L-arabinose isomerase, which transformants express the heterologous polynucleotides at a sufficient functional level to be effective to produce xylitol from arabinose. Production of xylitol is effected by contacting these recombinant microorganisms with a substrate comprising arabinose under conditions effective to produce xylitol from arabinose.
Mannitol is produced in a highly efficient fermentative method using NRRL B-30560, or in a biochemical method using mannitol dehydrogenase isolated from this strain. Fructose serves as the primary carbon substrate in both the fermentative and biochemical conversions, but important secondary carbon sources include glucose, maltose, mannose and galactose. Mannitol is useful in the food, pharmaceutical, and medicine industries as a sweet-tasting bodying and texturing agent.
Methods For Production Of Xylitol In Microorganisms
Paul Taylor - Arlington Heights IL, US Ian Fotheringham - Schaumburg IL, US Nathan Wymer - Peoria IL, US Badal Saha - Peoria IL, US David Demirjian - Hinsdale IL, US Yoshikiyo Sakaibara - Peoria IL, US Francis Michael Racine - Peoria IL, US
The invention provides biosynthetic routes to xylitol production that do not require pure -xylose for synthesis and that can utilize inexpensive substrates such as hemicellulose hydrolysates.
Method For Preparing High Conversion Syrups And Other Sweeteners
Joseph G. Zeikus - Okemos MI Badal C. Saha - East Lansing MI
Assignee:
Michigan Biotechnology Institute - Lansing MI
International Classification:
C12P 1922 C12N 926 C12R 1145
US Classification:
435 95
Abstract:
An improved method of preparing high maltose conversion syrups and other sweeteners from starch substrates comprises saccharifying the starch at higher temperatures than presently used with environmentally compatible thermostable. beta. -amylase and other thermostable enzymes.
Usda-Ars
Research Chemist and Lead Scientist
Michigan Biotechnology Institute Lansing Mi Oct 1985 - Jan 1992
Research Scientist
University of Maryland College Park Md Apr 1984 - Oct 1985
Faculty Research Associate
Bangladesh Agricultural University Mymensingh Dec 1974 - Mar 1979
Lecturer and Assistant Professor
Education:
Kyushu University 1979 - 1984
Doctorates, Doctor of Philosophy
Osaka University, Osaka, Japan 1977 - 1978
Dhaka University, Dhaka, Bangladesh 1966 - 1972
Master of Science, Masters, Bachelors, Bachelor of Science, Biochemistry
Skills:
Biochemistry Fermentation Biotechnology Microbiology Biofuels Molecular Biology Life Sciences Protein Chemistry Fermentation Technology Purification Protein Purification Hplc Chromatography Research Technology Transfer Chemistry Protein Expression Cell Culture Uv/Vis Cell Dna Bioinformatics Analytical Chemistry Pcr Assay Development Sds Page High Performance Liquid Chromatography Lifesciences Enzyme Kinetics Metabolic Engineering Enzyme Assays Proteomics Genomics Mass Spectrometry Gel Electrophoresis Fplc Lc Ms Fda High Throughput Screening Polymerase Chain Reaction Uv/Vis Spectroscopy