Ashley K Powell

Address:

9012 Gln Spg Dr, Dallas, TX 75243

VIN:

3VWRM71K48M014400

Make:

VOLKSWAGEN

Model:

JETTA

Year:

2008

Address:

5825 Tremont St APT B, Dallas, TX 75214

VIN:

3VWSF71K17M099013

Make:

VOLKSWAGEN

Model:

JETTA

Year:

2007

US Patent:

20090220979, Sep 3, 2009

Filed:

Dec 11, 2008

Appl. No.:

12/333213

Inventors:

Ronald W. Davis - Palo Alto CA, US
Stefanie S. Jeffrey - Los Altos Hills CA, US
Michael N. Mindrinos - Menlo Park CA, US
R. Fabian Pease - Stanford CA, US
Ashley Ann Powell - Mountain View CA, US
AmirAli Hajhossein Talasaz - Stanford CA, US

Assignee:

The Board of Trustees of the Leland Stanford Junior University - Palo Alto CA

International Classification:

C12N 13/00
C12M 1/42
C12Q 1/68
C12N 5/06

US Classification:

435 6, 4351731, 4353081, 435325

Abstract:

Described here is an automated robotic device that isolates circulating tumor cells (CTCs) or other biological structures with extremely high purity. The device uses powerful magnetic rods covered in removable plastic sleeves. These rods sweep through blood samples, capturing, e.g., cancer cells labeled with antibodies linked to magnetically responsive particles such as superparamagnetic beads. Upon completion of the capturing protocol, the magnetic rods undergo several rounds of washing, thereby removing all contaminating blood cells. The captured target cells are released into a final capture solution by removing the magnetic rods from the sleeves. Additionally, cells captured by this device show no reduced viability when cultured after capture. Cells are captured in a state suitable for genetic analysis. Also disclosed are methods for single cell analysis. Being robotic allows the device to be operated with high throughput.

US Patent:

20120045828, Feb 23, 2012

Filed:

Oct 28, 2011

Appl. No.:

13/284482

Inventors:

Ronald W. Davis - Palo Alto CA, US
Stefanie S. Jeffrey - Los Altos Hills CA, US
Michael N. Mindrinos - Menlo Park CA, US
R. Fabian Pease - Stanford CA, US
Ashley Ann Powell - Mountain View CA, US
AmirAli Hajhossein Talasaz - Menlo Park CA, US

Assignee:

The Board of Trustees of the Leland Stanford Junior University - Palo Alto CA

International Classification:

C12N 5/09
C12M 3/00

US Classification:

435325, 4353081

Abstract:

Described here is an automated robotic device that isolates circulating tumor cells (CTCs) or other biological structures with extremely high purity. The device uses powerful magnetic rods covered in removable plastic sleeves. These rods sweep through blood samples, capturing, e.g., cancer cells labeled with antibodies linked to magnetically responsive particles such as superparamagnetic beads. Upon completion of the capturing protocol, the magnetic rods undergo several rounds of washing, thereby removing all contaminating blood cells. The captured target cells are released into a final capture solution by removing the magnetic rods from the sleeves. Additionally, cells captured by this device show no reduced viability when cultured after capture. Cells are captured in a state suitable for genetic analysis. Also disclosed are methods for single cell analysis. Being robotic allows the device to be operated with high throughput.