Andrew C Gardner

US Patent:

20060199214, Sep 7, 2006

Filed:

May 4, 2006

Appl. No.:

11/417625

Inventors:

William Jack - Wenham MA, US
Andrew Gardner - Boston MA, US
Philip Buzby - Brockton MA, US
James DiMeo - Needham MA, US

International Classification:

C12Q 1/68
C07H 21/04
C12P 19/34
C12N 9/22
C12N 1/21
C12N 15/74

US Classification:

435006000, 435091100, 435252300, 435471000, 435199000, 536023200

Abstract:

The present invention is directed toward improving the efficiency of chain terminator incorporation by Family B archaeon DNA polymerases. Previously, the low efficiency of ddNTP, and more especially dye-labeled ddNTP, incorporation has limited the usefulness of this group of DNA polymerases in protocols requiring chain terminator incorporation.

US Patent:

20110045489, Feb 24, 2011

Filed:

Apr 20, 2009

Appl. No.:

12/988729

Inventors:

Andrew Gardner - Manchester MA, US
Lucia Greenough - Ipswich MA, US
William E. Jack - Wenham MA, US

Assignee:

NEW ENGLAND BIOLABS, INC. - Ipswich MA

International Classification:

C12Q 1/68
C12N 9/12
C12P 19/34

US Classification:

435 6, 435194, 435 915

Abstract:

Compositions and methods are provided that relate to a recombinant protein with DNA polymerase activity in which one or more amino acids are mutated compared with the corresponding wild type protein. The recombinant protein is capable of incorporating one or more modified nucleotides into a nucleic acid substrate with a specific activity greater than 200.

US Patent:

20130196327, Aug 1, 2013

Filed:

May 10, 2012

Appl. No.:

13/822724

Inventors:

Andrew Gardner - Manchester MA, US

Assignee:

NEW ENGLAND BIOLABS, INC. - Ipswich MA

International Classification:

C12N 9/12

US Classification:

435 611, 435194, 536 232, 4353201, 435243, 4352351, 435 912

Abstract:

Compositions and methods are described to modify Family B DNA polymerases that contain residual exonuclease activity that interferes with sequencing techniques and with detection of single nucleotide polymorphisms. The compositions are mutant proteins with reduced exonuclease activity compared with presently available “exo” polymerases, and a sensitive screening assay that enables an assessment of exonuclease activity of any synthetic DNA polymerase.

US Patent:

20220307081, Sep 29, 2022

Filed:

May 26, 2022

Appl. No.:

17/825346

Inventors:

- Ipswich MA, US
Andrew F. Gardner - Manchester MA, US

Assignee:

New England Biolabs, Inc. - Ipswich MA

International Classification:

C12Q 1/6855
C12N 9/22
C12N 9/00
C12Q 1/6874

Abstract:

Provided herein, among other things, are various in vitro methods that involve cleaving dsDNA molecules that comprise a mismatched nucleotide using EndoMS. In some embodiments, the method may comprise ligating a T-tailed double-stranded adapter to A-tailed double-stranded fragments of nucleic acid to produce ligation products that comprise adapter-ligated fragments and double-stranded adapter dimers that comprise a T:T mismatch at the ligation junction and cleaving both strands of the adapter dimers using EndoMS.

US Patent:

20210164030, Jun 3, 2021

Filed:

Jun 11, 2018

Appl. No.:

16/616631

Inventors:

- Ipswich MA, US
Andrew F. Gardner - Manchester MA, US

Assignee:

New England Biolabs, Inc. - Ipswich MA

International Classification:

C12Q 1/6855
C12Q 1/6874
C12N 9/22
C12N 9/00

Abstract:

Provided herein, among other things, are various in vitro methods that involve cleaving dsDNA molecules that comprise a mismatched nucleotide using EndoMS. In some embodiments, the method may comprise ligating a T-tailed double-stranded adapter to A-tailed double-stranded fragments of nucleic acid to produce ligation products that comprise adapter-ligated fragments and double-stranded adapter dimers that comprise a T:T mismatch at the ligation junction and cleaving both strands of the adapter dimers using EndoMS.

US Patent:

20200325533, Oct 15, 2020

Filed:

Aug 21, 2018

Appl. No.:

16/758190

Inventors:

- Ipswich MA, US
Vladimir Potapov - Auburndale MA, US
Jennifer Ong - Salem MA, US
Laurence Ettwiller - Beverly MA, US
Lixin Chen - Beverly MA, US
Thomas C. Evans, Jr. - Topsfield MA, US
Andrew F. Gardner - Manchester MA, US

Assignee:

New England Biolabs, Inc. - Ipswich MA

International Classification:

C12Q 1/6869
C12Q 1/6806

Abstract:

Providing herein, among other things, is a method comprising incubating a double-stranded nucleic acid having a nick with a nick translating activity, a ligase, and a nucleotide mix comprising at least one modified nucleotide, to generate a product comprising a patch of a newly synthesized strand of a duplex nucleic acid containing a plurality of modified nucleoside monophosphates that are at or adjacent to the site of the nick. In some embodiments, the method may be used to map damaged nucleoside monophosphates in a nucleic acid. Compositions and kits for use in performing the method are also provided.

US Patent:

20180355328, Dec 13, 2018

Filed:

Aug 20, 2018

Appl. No.:

16/105502

Inventors:

- Ipswich MA, US
Luo Sun - Hamilton MA, US
Thomas C. Evans, JR. - Topsfield MA, US
Theodore B. Davis - Boxford MA, US
Andrew F. Gardner - Manchester MA, US

Assignee:

New England Biolabs, Inc. - Ipswich MA

International Classification:

C12N 9/12
C12N 9/96
C12N 9/22
C12N 9/20
C12N 15/66
C12N 15/10
C12P 19/34
C12N 15/64

Abstract:

This disclosure provides, among other things, a composition comprising: comprising a fusion protein comprising: (a) a DNA polymerase; and (b) a heterologous sequence-specific DNA binding domain. A method for copying a DNA template, as well as a kit for performing the same, are also described.

US Patent:

20180216098, Aug 2, 2018

Filed:

Aug 27, 2015

Appl. No.:

15/506050

Inventors:

- Ipswich MA, US
Luo Sun - Hamilton MA, US
Thomas C. Evans, Jr. - Topsfield MA, US
Theodore B. Davis - Boxford MA, US
Andrew Gardner - Manchester MA, US

Assignee:

New England Biolabs, Inc. - Ipswich MA

International Classification:

C12N 15/10
C12N 15/64
C12N 15/66
C12N 9/12
C12N 9/96

Abstract:

This disclosure provides, among other things, a composition comprising: a 5′ exonuclease; a strand-displacing polymerase; and optionally a single strand DNA binding protein and/or a ligase. A method for polynucleotide assembly to form a synthon, as well as a kit for performing the same, are also described.

Author:

Andrew B. Gardner

ISBN #:

0399508058

Author:

Andrew B. Gardner

ISBN #:

0448133229