Andrea E Murmann

US Patent:

20200299694, Sep 24, 2020

Filed:

Mar 19, 2020

Appl. No.:

16/824589

Inventors:

- Evanston IL, US
Andrea E. Murmann - Chicago IL, US

Assignee:

Northwestern University - Evanston IL

International Classification:

C12N 15/113
C12N 15/86

Abstract:

Disclosed are components and methods for producing toxic RNAs and/or extracellular vesicles comprising the toxic RNAs in cells that are resistant to the toxic RNAs, which toxic RNAs may include toxic shRNA/siRNA and/or toxic pre-miRNA/miRNA. The disclosed components may include engineered cells that can be utilized to express the toxic RNAs, in which the engineered cells do not express one or more genes that are required for processing the toxic RNAs for RNA interference (RNAi) and/or one or more genes that are required for executing RNAi. The toxic RNAs and/or extracellular vesicles comprising the toxic RNAs may be utilized in methods for treating diseases and disorders through RNAi.

US Patent:

20200299697, Sep 24, 2020

Filed:

Mar 19, 2020

Appl. No.:

16/824592

Inventors:

- Evanston IL, US
Andrea E. Murmann - Chicago IL, US

Assignee:

Northwestern University - Evanston IL

International Classification:

C12N 15/113
A61P 35/00

Abstract:

Disclosed are polynucleotides, compositions, and methods related to RNA interference (RNAi). Particular disclosed are toxic RNAi active seed sequences and methods of using toxic RNAi active sequences for killing cancer cells. The disclosed toxic RNAi active seed sequences preferentially target and inhibit the expression of multiple essential genes for cell survival and/or growth through a process called “death-induced by survival gene elimination” or “DISE.” The disclosed toxic RNAi active seed sequences may be referred to as “dual activity super toxic RNAi active dsRNAs” that include a toxic first strand and a toxic second strand that is complementary to the toxic first strand. As such, the disclosed dsRNAs may be expressed as shRNAs which are processed for RNA interference (RNAi) and either of the toxic first strand and the toxic second strand can function as a guide strand to initiate RNAi.

US Patent:

20180320187, Nov 8, 2018

Filed:

Feb 20, 2018

Appl. No.:

15/900392

Inventors:

- Evanston IL, US
William E. Putzbach - Lombard IL, US
Andrea E. Murmann - Chicago IL, US
Monal Patel - Des Plaines IL, US
Quan Gao - Chicago IL, US

Assignee:

Northwestern University - Evanston IL

International Classification:

C12N 15/113
A61K 47/69
A61K 47/64
A61K 31/713
A61P 35/00

Abstract:

Disclosed are polynucleotides, compositions, and methods related to RNA interference (RNAi). The disclosed polynucleotides, compositions, and methods may be utilized for treating diseases and disorders through RNAi. Particular disclosed are toxic RNAi active seed sequences and methods of using toxic RNAi active sequences for killing cancer cells. The disclosed toxic RNAi active seed sequences preferentially target and inhibit the expression of multiple essential genes for cell survival and/or growth through a process called “death-induced by survival gene elimination” or “DISE.”

US Patent:

20180251762, Sep 6, 2018

Filed:

Feb 20, 2018

Appl. No.:

15/899974

Inventors:

- Evanston IL, US
Andrea E. Murmann - Chicago IL, US

Assignee:

Northwestern University - Evanston IL

International Classification:

C12N 15/113
A61P 35/00

Abstract:

Disclosed are compositions and methods related to RNA interference (RNAi) and the use of RNAi active sequence for treating diseases and disorders. Particular disclosed are toxic RNAi active sequences such as siRNA and shRNA for killing cancer cells. The disclosed toxic RNAi active sequences typically include trinucleotide repeats and preferentially target the expression of multiple essential genes for cell survival and/or growth.