Alon Singer

US Patent:

20130203610, Aug 8, 2013

Filed:

Mar 30, 2011

Appl. No.:

13/638455

Inventors:

Amit Meller - Brookline MA, US
Alon Singer - Brighton MA, US

Assignee:

TRUSTEES OF BOSTON UNIVERSITY - Boston MA

International Classification:

C12Q 1/68

US Classification:

506 6, 506 16

Abstract:

Provided herein is a library that comprises a plurality of molecular beacons (MBs), each MB having a detectable label, a detectable label blocker and a modifier group. The library is used in conjunction with nanopore unzipping-dependent sequencing of nucleic acids.

US Patent:

20130256118, Oct 3, 2013

Filed:

May 11, 2011

Appl. No.:

13/697067

Inventors:

Amit Meller - Brookline MA, US
Zhiping Weng - Wellesley MA, US
Alon Singer - Brighton MA, US
Benjamin McNally - Brighton MA, US

Assignee:

TRUSTEES OF BOSTON UNIVERSITY - Boston MA

International Classification:

G01N 33/487
C12Q 1/68

US Classification:

20415821, 204543, 204519

Abstract:

Described are techniques for optical detection of single molecule signals from a nanopore array for analysis of nucleic acid sequences. These techniques are useful for rapid multiplexed DNA sequencing.

US Patent:

20120276530, Nov 1, 2012

Filed:

Aug 24, 2010

Appl. No.:

13/391695

Inventors:

Amit Meller - Brookline MA, US
Meni Wanunu - Chestnut Hill MA, US
Heiko Kuhn - Seeheim-Jugenheim, DE
Alon Singer - Brighton MA, US
Will Morrison - Allston MA, US

Assignee:

TRUSTEES OF BOSTON UNIVERSITY - Boston MA

International Classification:

G01N 27/26
B82Y 5/00

US Classification:

435 611, 436501, 435 5, 435 615, 977781, 977920

Abstract:

Embodiments disclosed herein relate to a method of detecting specific DNA sequences and the application of this method in the detection of pathogens, viruses, drug-resistant pathogens, genomic variations associated with disease/disorder susceptibility etc. based on specific signature sequences unique to the pathogens, viruses, drug-resistant pathogens or genomic variations. The method can also be used to distinguish a pool of same-sized dsDNA on the basis of sequence differences. The method uses non-optically labeled bis-PNA and/or gamma-PNA probes to tag specific target sequences for identification by solid-state nanopores.

US Patent:

20170218434, Aug 3, 2017

Filed:

Apr 14, 2017

Appl. No.:

15/488204

Inventors:

- Marlborough MA, US
Alon Singer - Brighton MA, US

International Classification:

C12Q 1/68

Abstract:

The present disclosure provides for compositions of γPNA probes. Additionally, the present disclosure provide for methods and kits using γPNA probes for the diagnosis of sepsis.

US Patent:

20150099657, Apr 9, 2015

Filed:

May 17, 2013

Appl. No.:

14/402028

Inventors:

- Marlborough MA, US
Alon Singer - Brighton MA, US

International Classification:

C12Q 1/68

US Classification:

506 9, 506 18

Abstract:

The present disclosure provides for composition of γPNA probes. Additionally, the present disclosure provide for methods and kits using γPNA probes for the prognosis of sepsis. In some embodiments, wherein the γPNA capture probes and γPNA reporter probes comprise one or more functional moiety selected from the group consisting of: a binding molecule, a spacer group, a linker group, a hydrophobicity-changing group, a charge-inducing group, and a structural charge-inducing group.