Alan Carol Rosenberg

US Patent:

D488295, Apr 13, 2004

Filed:

Feb 3, 2003

Appl. No.:

29/175281

Inventors:

Alan Rosenberg - Westport CT
Steven Podhaizer - Holmdel NJ

Assignee:

R S Sales Co., Inc.

International Classification:

0299

US Classification:

D 2961

US Patent:

56934890, Dec 2, 1997

Filed:

Jun 14, 1994

Appl. No.:

8/259560

Inventors:

F. William Studier - Stony Brook NY
Parichehre Davanloo - Basel, CH
Alan H. Rosenberg - Setauket NY
Barbara A. Moffatt - Waterloo, CA
John J. Dunn - Bellport NY

Assignee:

Associated Universities, Inc. - Washington DC

International Classification:

C12N 1567
C12N 1500
C12N 1570
C12N 121

US Classification:

435 691

Abstract:

This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the R7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells.

US Patent:

58693203, Feb 9, 1999

Filed:

Jan 15, 1997

Appl. No.:

8/784201

Inventors:

F. William Studier - Stony Brook NY
Parichehre Davanloo - Basel, CH
Alan H. Rosenberg - Setauket NY
Barbara A. Moffatt - Waterloo, CA
John J. Dunn - Bellport NY

Assignee:

Brookhaven Science Associates LLC - Upton NY

International Classification:

C12N 121
C12N 1534
C12N 1563
C12N 1570

US Classification:

43525233

Abstract:

This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the R7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells.

US Patent:

57669058, Jun 16, 1998

Filed:

Jun 17, 1996

Appl. No.:

8/664449

Inventors:

F. William Studier - Stony Brook NY
Alan H. Rosenberg - Setauket NY

Assignee:

Associated Universities Inc. - Washington DC

International Classification:

C12N 1509
C12Q 170
A61K 3900

US Classification:

4351723

Abstract:

Disclosed are display vectors comprising DNA encoding a portion of a structural protein from a cytoplasmic bacteriophage, joined covalently to a protein or peptide of interest. Exemplified are display vectors wherein the structural protein is the T7 bacteriophage capsid protein. More specifically, in the exemplified display vectors the C-terminal amino acid residue of the portion of the capsid protein is joined to the N-terminal residue of the protein or peptide of interest. The portion of the T7 capsid protein exemplified comprises an N-terminal portion corresponding to form 10B of the T7 capsid protein. The display vectors are useful for high copy number display or lower copy number display (with larger fusion). Compositions of the type described herein are useful in connection with methods for producing a virus displaying a protein or peptide of interest.

US Patent:

49524968, Aug 28, 1990

Filed:

Dec 29, 1986

Appl. No.:

7/002408

Inventors:

F. William Studier - Stony Brook NY
Parichehre Davanloo - Basel, CH
Alan H. Rosenberg - Setauket NY
Barbara A. Moffatt - East Lansing MI
John J. Dunn - Bellport NY

Assignee:

Associated Universities, Inc. - Washington DC

International Classification:

C12P 1934
C12N 1500
C12N 700
C12N 912

US Classification:

435 91

Abstract:

This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the T7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells.

Author:

Alan L. Rosenberg

ISBN #:

0668045191

Author:

Alan L. Rosenberg

ISBN #:

0668045221

Author:

Alan Rosenberg

ISBN #:

0275929485

Author:

Alan Rosenberg

ISBN #:

0391039253

Author:

Alan Rosenberg

ISBN #:

0391040154

Author:

Alan Rosenberg

ISBN #:

0814755011

Author:

Alan Rosenberg

ISBN #:

0814756085

Author:

Alan Rosenberg

ISBN #:

0816633789