- Ithaca NY, US Abdullah Ozer - Ithaca NY, US John Lis - Ithaca NY, US
International Classification:
C12N 15/115 C12Q 1/6844 C12Q 1/6869 G01N 33/543
Abstract:
The present application relates to a method for analyzing a molecular target in a sample. The method involves providing an aptamer, wherein the aptamer is a high affinity binding partner to at least a portion of the molecular target in the sample. The aptamer is contacted with the sample containing the molecular target under conditions effective for the molecular target and aptamer to bind to each other. The molecular target is separated from the sample to form a molecular target enriched sample. The separated molecular target of the enriched sample is then analyzed.
Rna Aptamer Isolation Via Dual-Cycle (Rapid) Selection
- Ithaca NY, US David R. Latulippe - Paris, CA John T. Lis - Ithaca NY, US Abdullah Ozer - Vestal NY, US Kylan Szeto - Ithaca NY, US
Assignee:
CORNELL UNIVERSITY - Ithaca NY
International Classification:
C12N 15/10 G06F 19/28 G06F 19/00 C12N 15/115
Abstract:
The present invention relates to a method for selecting an aptamer for a target molecule. The method involves providing a random oligonucleotide library comprising a plurality of unique random sequence oligonucleotides; providing a target mixture comprising at least one target molecule; and subjecting the random oligonucleotide library and the target mixture to at least one round of an aptamer isolation protocol to yield at least one aptamer for the target molecule, wherein a round of the aptamer isolation protocol comprises at least one selection cycle followed by an amplification cycle. The present invention also relates to systems and devices for implementing or performing the method of the present invention. The present invention further relates to using the method to isolate aptamers for high-throughput sequencing analysis and other aptamer analysis protocols.
Multiplexed Microcolumn Devices And Processes For Selection Of Nucleic Acid Aptamers
- Ithaca NY, US David R. Latulippe - Ithaca NY, US John T. Lis - Ithaca NY, US Abdullah Ozer - Vestal NY, US Kylan Szeto - Ithaca NY, US
Assignee:
CORNELL UNIVERSITY - Ithaca NY
International Classification:
G01N 33/53
Abstract:
The present invention relates to a microcolumn device for selecting nucleic acid aptamers for single and multiple target molecules, as well as a method for making the microcolumn device. The present invention also relates to a system for selecting nucleic acid aptamers for single and multiple target molecules. The present invention further relates to methods of using the microcolumn device for selecting nucleic acid aptamers for multiple target molecules. Kits that include one or more microcolumn device and/or system of the present invention are also disclosed.
Programmable And Reconfigurable Microcolumn Affinity Chromatograpy Device, System, And Methods Of Use Thereof
- Ithaca NY, US Kylan SZETO - Ithaca NY, US Sarah REINHOLT - Ithaca NY, US John T. LIS - Ithaca NY, US Abdullah OZER - Vestal NY, US
Assignee:
CORNELL UNIVERSITY - Ithaca NY
International Classification:
C12N 15/10 B01D 15/22 B01D 15/38
Abstract:
The present invention generally relates to microcolumn affinity chromatography devices, systems that include the microcolumn affinity chromatography devices of the present disclosure, methods of using the devices and the systems of the present disclosure, and methods of making the devices and the systems of the present disclosure. In certain embodiments, the microcolumn affinity chromatography device is suitable for conducting affinity chromatography in multiple microcolumns in parallel and/or in series.
John T. LIS - Ithaca NY, US Abdullah OZER - Vestal NY, US Jacob M. TOME - Ithaca NY, US
Assignee:
CORNELL UNIVERSITY - Ithaca NY
International Classification:
C12Q 1/68
US Classification:
506 9, 435 611
Abstract:
The present invention is directed to a method for detecting an interaction between a ribonucleic acid (RNA) molecule and a second molecule. This method involves providing a nucleic acid construct that contains a promoter sequence, a nucleotide sequence encoding the RNA molecule, and an RNA polymerase blocking site. The nucleotide sequence encoding the RNA molecule is transcribed in vitro to produce an RNA transcript corresponding to the RNA molecule. Transcription is halted by the RNA polymerase blocking site under conditions effective for the RNA transcript to remain tethered to the nucleic acid construct. The tethered RNA transcript is contacted with the second molecule and any interaction between the tethered RNA transcript and the second molecule is detecting and identified based on said contacting.
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