Aaron E Carnes

US Patent:

20070254342, Nov 1, 2007

Filed:

Aug 16, 2005

Appl. No.:

11/573825

Inventors:

Aaron Carnes - Lincoln NE, US
James Williams - Lincoln NE, US

Assignee:

NATURE TECHNOLOGY CORP. - Lincoln NE

International Classification:

C12P 19/34
C12N 15/64

US Classification:

435091200, 435091100

Abstract:

Improvements in plasmid DNA production technology are needed to insure the economic feasibility of future DNA vaccines and DNA therapeutics. General methods are described, by means of which it is possible to dramatically increase plasmid DNA productivity in a fermentor. These processes feature Fed-batch fermentation strategies, combined with novel growth and induction phase temperature shifts.

US Patent:

20080318283, Dec 25, 2008

Filed:

Jan 31, 2007

Appl. No.:

12/162689

Inventors:

Aaron E. Carnes - Lincoln NE, US

Assignee:

NATURE TECHNOLOGY CORPORATION - Lincoln NE

International Classification:

C12N 15/64

US Classification:

435 914

Abstract:

A continuous process is described for the production of microbial plasmid DNA for use in biopharmaceutical and biotechnological applications. The process consists of: first growing microbial cells containing a plasmid at a reduced temperature in a continuous stage; followed by a second plasmid induction continuous culture stage with an increased temperature, with a residence time that allows accumulation of the plasmid product. A hold step at a reduced temperature after fermentation further increases the yield of plasmid product. The method enables production of a large quantity of highly purified plasmid DNA from a small bioreactor over time.

US Patent:

20100184157, Jul 22, 2010

Filed:

May 22, 2008

Appl. No.:

12/601504

Inventors:

James A. Williams - Lincoln NE, US
Clague P. Hodgson - Omaha NE, US
Aaron E. Carnes - Lincoln NE, US

Assignee:

NATURE TECHNOLOGY CORPORATION - Lincoln NE

International Classification:

C12N 15/64
C12N 1/21

US Classification:

435 914, 4352523, 43525233

Abstract:

General methods and strains of bacteria are described, that dramatically simplify and streamline plasmid DNA production. In one preferred embodiment, endolysin mediated plasmid extraction combined with flocculation mediated removal of cell debris and host nucleic acids achieves increased yield and purity with simplified downstream purification and reduced waste streams, thus reducing production costs.

US Patent:

20110281304, Nov 17, 2011

Filed:

May 16, 2011

Appl. No.:

13/108294

Inventors:

Aaron E. CARNES - Lincoln NE, US
James A. Williams - Lincoln NE, US

Assignee:

Nature Technology Corp. - Lincoln NE

International Classification:

C12P 19/34

US Classification:

435 911

Abstract:

Improvements in plasmid DNA production technology are needed to insure the economic feasibility of future DNA vaccines and DNA therapeutics. General methods are described, by means of which it is possible to dramatically increase plasmid DNA productivity in a fermentor. These processes feature fed-batch fermentation strategies, combined with novel growth and induction phase temperature shifts.

US Patent:

20160355827, Dec 8, 2016

Filed:

Feb 3, 2015

Appl. No.:

14/612968

Inventors:

- Lincoln NE, US
CLAGUE P. HODGSON - OMAHA NE, US
AARON E. CARNES - LINCOLN NE, US

International Classification:

C12N 15/64
C12N 15/10

Abstract:

General methods and strains of bacteria are described that dramatically simplify and streamline plasmid DNA production. In one preferred embodiment, endolysin mediated plasmid extraction combined with flocculation mediated removal of cell debris and host nucleic acids achieves increased yield and purity with simplified downstream purification and reduced waste streams, thus reducing production costs.

US Patent:

20150225732, Aug 13, 2015

Filed:

Feb 3, 2015

Appl. No.:

14/612960

Inventors:

- Lincoln NE, US
AARON E. CARNES - LINCOLN NE, US

International Classification:

C12N 15/73

Abstract:

General methods and strains of bacteria are described that dramatically simplify and streamline plasmid DNA production. In one preferred embodiment, endolysin mediated plasmid extraction combined with flocculation mediated removal of cell debris and host nucleic acids achieves increased yield and purity with simplified downstream purification and reduced waste streams, thus reducing production costs.

US Patent:

20150203854, Jul 23, 2015

Filed:

Feb 3, 2015

Appl. No.:

14/612952

Inventors:

- Lincoln NE, US
AARON E. CARNES - LINCOLN NE, US

International Classification:

C12N 15/64

Abstract:

General methods and strains of bacteria are described that dramatically simplify and streamline plasmid DNA production. In one preferred embodiment, endolysin mediated plasmid extraction combined with flocculation mediated removal of cell debris and host nucleic acids achieves increased yield and purity with simplified downstream purification and reduced waste streams, thus reducing production costs.